Although honey bees can be killed in place inside buildings by using pesticides that are labelled for killing bees inside of structures, this removal option often leads to undesirable consequences.(Note: These chemicals are available only to licensed pest control operators).

If the colony is well established, there are further issues associated with killing the colony. Unattended brood can also rot and become very odorous. Unattended honey stores can absorb moisture and ferment, creating gas that causes the capping holding honey in the cells to burst. Honey then seeps through the drywall, leading to large amounts of clean-up and expensive replacement. If pesticides were used to kill the bees, then the honey, wax and, dead bees are contaminated and must be handled as hazardous waste.

A better procedure than applying insecticides, especially if you have a beekeeper who is willing to help, may be to eliminate the bees without killing them. First the beekeeper will need to locate the nest by tapping the wall and listening for the hum of the colony. Some beekeepers rely on stethoscopes to find the edges of the nest. Others drill extremely small holes in the wall and insert a fine wire to find the periphery of the nest. To take honey bees and their combs from the nesting spot requires opening a fairly large hole in some portion of the building. That is best done by a professional contractor so that the hole can be easily closed after the bees are removed.